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sst 14 h-1490  (Bachem)

 
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    Bachem sst 14 h-1490
    Sst 14 H 1490, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sst+14/sst+14/pmc10542913-11-0-3
    Average 90 stars, based on 1 article reviews
    sst 14 h-1490 - by Bioz Stars, 2026-10
    90/100 stars

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    Virus:

    Article Title: Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9.Ef1a.fDIO.GCaMP6f (Menegas et al.)49 Addgene #118273 AAV9.CAG.FLEX.TdTomato (Oh et al.)50 Addgene #51503 AAV5-EF1a-DIO-hChR2(H134R)-eYFPWPRE-HGHpA Karl Deisseroth Addgene #20298 Chemicals, peptides, and recombinant proteins Picrotoxin Hello Bio HB0506 CGP 55845 Tocris 1248 SST 14 Bachem H-1490 Cyclosomatostatin (cyclo-SST) Abcam ab141211 Octreotide Acetate Sigma Aldrich PHR 1880 Tetrodotoxin (TTX) Abcam ab120054 2,2,2-Tribromoethanol (Avertin) Thermo Fisher Scientific A18706.14 Isoflurane Piramal Pharma Limited NDC: 66794-013-25 Ketoprofen (100 mg/ml injectable solution) Manufacturer: Zoetis Supplier: Midwest Veterinary Supply 275.22120.3 Bupivacaine HCl Manufacturer: Hospira Supplier: Midwest Veterinary Supply 191.26505.3 4% Paraformaldehyde Thermo Fisher Scientific J19943.K2 Kynurenic acid Sigma K3376 Immu-Mount Thermo Fisher Scientific 9990402 DAPI Fluoromount-G Southern Biotech 0100–20 Deposited data Code and Data This study Dryad: https://datadryad.org/stash/share/ ouwbAlBUHoEafVxikaupvUOuh Sn3wUHZYc096IYwM2c Experimental models: Organisms/strains C57BL/6J Jackson Labs #000664 SST-IRES-Cre+/ Jackson Labs #013044 VIP::Cre Jackson Labs #010908 SST::Flp Jackson Labs #028579 Df(16)A+/ (Stark et al.)51 N/A Software and algorithms Clampex (Clampfit and pClamp 11) Molecular Devices https://www.moleculardevices.com/ Biorender Biorender https://www.biorender.com/ Prism Graphpad https://www.graphpad.com/ MATLAB The Math Works, Inc. https://www.mathworks.com/ products/matlab.html Python (Van Rossum)52 N/A Deep Lab Cut (Mathis et al.)53 https://github.com/DeepLabCut/ DeepLabCut Other Vibrating Microtome VF-300-0Z Precisionary Instruments N/A Leica Vibratome VS. 1200 Leica N/A 7000 smz2 Vibratome Campden Instruments N/A Narishige PC-100 Puller Narishige Group N/A (Continued on next page) Cell Reports 42, 112976, August 29, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Borosilicate Glass Capillaries Warner Instruments 64–0772 470-nm LED pE-100 Cool LED pE-100 Contemporary Ortho-Jet Powder (Bilateral Cannula) Lang Dental Ref. 1530 Ortho-Jet Liquid (Bilateral Cannula) Lang Dental Ref. 1304 Dental Cement (Fiber Photometry) Unifast Trad N/A Vetbond 3M 1469Sb ICV Bilateral Internal Cannula P1 Technologies 8IC235ISPCXC ICV Bilateral Guide Cannula P1 Technologies 8IC235G10XXC ICV Bilateral Dummy Cannula P1 Technologies 8IC235DCSPCC Bilateral Cannula Dust Cap P1 Technologies 8K0000303DC1 Stereotaxic frame (Bilateral Cannula/ Optogenetic Release): Drill and Microinjector Robot, Mouse/Rat Stoelting SKU: 51705 Stereotaxic frame (Fiber Photometry) Kopf Instruments Model 942 1 mL Neuros syringe Hamilton 65458–01 5 mL Removable Needle Syringe Hamilton #7634-01 Syringe Pump Stoelting #53311 miniature screws Antrin Miniature Specialities AMS90/1B Fiber Optic Cannula (200 mm core, 0.39 NA) ThorLabs CFMC12L05 473-nm Laser Ready Lasers MBL-III-473-100mW Noise Eeater ThorLabs NEL01 Kinematic Flourescence Filter Cube ThorLabs DFM1 Dual-Edge Dichoric Mirror Chroma ZT488/561rpc FC/PC Fiber Coupler Thorlabs PAF2-A4A Fiber Patchcord (200 mm core, 0.39 NA) ThorLabs M72L05 Fiberoptic Rotary Joint Doric Lenses FRJ_1x1_FC-FC Mating Sleeve Precision Fiber Products SM-CS1140S Dual-band Emission Filter Chroma ZET488/561m Fiber Coupler ThorLabs PAF2S-11A Anti-reflection-coated Fiber ThorLabs M200L02S-A Spectrometer Ocean Insight QE Pro BX63 Upright Microscope Olympus N/A CMS GmbH CMOS fitted Microscope Leica Microsystems N/A LSM 800 Confocal Microscope Zeiss N/A Waveform Generator Sanworks Pulse Pal v2 Blackfly S USB3 Camera FLIR N/A

    Recombinant:

    Article Title: Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9.Ef1a.fDIO.GCaMP6f (Menegas et al.)49 Addgene #118273 AAV9.CAG.FLEX.TdTomato (Oh et al.)50 Addgene #51503 AAV5-EF1a-DIO-hChR2(H134R)-eYFPWPRE-HGHpA Karl Deisseroth Addgene #20298 Chemicals, peptides, and recombinant proteins Picrotoxin Hello Bio HB0506 CGP 55845 Tocris 1248 SST 14 Bachem H-1490 Cyclosomatostatin (cyclo-SST) Abcam ab141211 Octreotide Acetate Sigma Aldrich PHR 1880 Tetrodotoxin (TTX) Abcam ab120054 2,2,2-Tribromoethanol (Avertin) Thermo Fisher Scientific A18706.14 Isoflurane Piramal Pharma Limited NDC: 66794-013-25 Ketoprofen (100 mg/ml injectable solution) Manufacturer: Zoetis Supplier: Midwest Veterinary Supply 275.22120.3 Bupivacaine HCl Manufacturer: Hospira Supplier: Midwest Veterinary Supply 191.26505.3 4% Paraformaldehyde Thermo Fisher Scientific J19943.K2 Kynurenic acid Sigma K3376 Immu-Mount Thermo Fisher Scientific 9990402 DAPI Fluoromount-G Southern Biotech 0100–20 Deposited data Code and Data This study Dryad: https://datadryad.org/stash/share/ ouwbAlBUHoEafVxikaupvUOuh Sn3wUHZYc096IYwM2c Experimental models: Organisms/strains C57BL/6J Jackson Labs #000664 SST-IRES-Cre+/ Jackson Labs #013044 VIP::Cre Jackson Labs #010908 SST::Flp Jackson Labs #028579 Df(16)A+/ (Stark et al.)51 N/A Software and algorithms Clampex (Clampfit and pClamp 11) Molecular Devices https://www.moleculardevices.com/ Biorender Biorender https://www.biorender.com/ Prism Graphpad https://www.graphpad.com/ MATLAB The Math Works, Inc. https://www.mathworks.com/ products/matlab.html Python (Van Rossum)52 N/A Deep Lab Cut (Mathis et al.)53 https://github.com/DeepLabCut/ DeepLabCut Other Vibrating Microtome VF-300-0Z Precisionary Instruments N/A Leica Vibratome VS. 1200 Leica N/A 7000 smz2 Vibratome Campden Instruments N/A Narishige PC-100 Puller Narishige Group N/A (Continued on next page) Cell Reports 42, 112976, August 29, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Borosilicate Glass Capillaries Warner Instruments 64–0772 470-nm LED pE-100 Cool LED pE-100 Contemporary Ortho-Jet Powder (Bilateral Cannula) Lang Dental Ref. 1530 Ortho-Jet Liquid (Bilateral Cannula) Lang Dental Ref. 1304 Dental Cement (Fiber Photometry) Unifast Trad N/A Vetbond 3M 1469Sb ICV Bilateral Internal Cannula P1 Technologies 8IC235ISPCXC ICV Bilateral Guide Cannula P1 Technologies 8IC235G10XXC ICV Bilateral Dummy Cannula P1 Technologies 8IC235DCSPCC Bilateral Cannula Dust Cap P1 Technologies 8K0000303DC1 Stereotaxic frame (Bilateral Cannula/ Optogenetic Release): Drill and Microinjector Robot, Mouse/Rat Stoelting SKU: 51705 Stereotaxic frame (Fiber Photometry) Kopf Instruments Model 942 1 mL Neuros syringe Hamilton 65458–01 5 mL Removable Needle Syringe Hamilton #7634-01 Syringe Pump Stoelting #53311 miniature screws Antrin Miniature Specialities AMS90/1B Fiber Optic Cannula (200 mm core, 0.39 NA) ThorLabs CFMC12L05 473-nm Laser Ready Lasers MBL-III-473-100mW Noise Eeater ThorLabs NEL01 Kinematic Flourescence Filter Cube ThorLabs DFM1 Dual-Edge Dichoric Mirror Chroma ZT488/561rpc FC/PC Fiber Coupler Thorlabs PAF2-A4A Fiber Patchcord (200 mm core, 0.39 NA) ThorLabs M72L05 Fiberoptic Rotary Joint Doric Lenses FRJ_1x1_FC-FC Mating Sleeve Precision Fiber Products SM-CS1140S Dual-band Emission Filter Chroma ZET488/561m Fiber Coupler ThorLabs PAF2S-11A Anti-reflection-coated Fiber ThorLabs M200L02S-A Spectrometer Ocean Insight QE Pro BX63 Upright Microscope Olympus N/A CMS GmbH CMOS fitted Microscope Leica Microsystems N/A LSM 800 Confocal Microscope Zeiss N/A Waveform Generator Sanworks Pulse Pal v2 Blackfly S USB3 Camera FLIR N/A

    Software:

    Article Title: Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV9.Ef1a.fDIO.GCaMP6f (Menegas et al.)49 Addgene #118273 AAV9.CAG.FLEX.TdTomato (Oh et al.)50 Addgene #51503 AAV5-EF1a-DIO-hChR2(H134R)-eYFPWPRE-HGHpA Karl Deisseroth Addgene #20298 Chemicals, peptides, and recombinant proteins Picrotoxin Hello Bio HB0506 CGP 55845 Tocris 1248 SST 14 Bachem H-1490 Cyclosomatostatin (cyclo-SST) Abcam ab141211 Octreotide Acetate Sigma Aldrich PHR 1880 Tetrodotoxin (TTX) Abcam ab120054 2,2,2-Tribromoethanol (Avertin) Thermo Fisher Scientific A18706.14 Isoflurane Piramal Pharma Limited NDC: 66794-013-25 Ketoprofen (100 mg/ml injectable solution) Manufacturer: Zoetis Supplier: Midwest Veterinary Supply 275.22120.3 Bupivacaine HCl Manufacturer: Hospira Supplier: Midwest Veterinary Supply 191.26505.3 4% Paraformaldehyde Thermo Fisher Scientific J19943.K2 Kynurenic acid Sigma K3376 Immu-Mount Thermo Fisher Scientific 9990402 DAPI Fluoromount-G Southern Biotech 0100–20 Deposited data Code and Data This study Dryad: https://datadryad.org/stash/share/ ouwbAlBUHoEafVxikaupvUOuh Sn3wUHZYc096IYwM2c Experimental models: Organisms/strains C57BL/6J Jackson Labs #000664 SST-IRES-Cre+/ Jackson Labs #013044 VIP::Cre Jackson Labs #010908 SST::Flp Jackson Labs #028579 Df(16)A+/ (Stark et al.)51 N/A Software and algorithms Clampex (Clampfit and pClamp 11) Molecular Devices https://www.moleculardevices.com/ Biorender Biorender https://www.biorender.com/ Prism Graphpad https://www.graphpad.com/ MATLAB The Math Works, Inc. https://www.mathworks.com/ products/matlab.html Python (Van Rossum)52 N/A Deep Lab Cut (Mathis et al.)53 https://github.com/DeepLabCut/ DeepLabCut Other Vibrating Microtome VF-300-0Z Precisionary Instruments N/A Leica Vibratome VS. 1200 Leica N/A 7000 smz2 Vibratome Campden Instruments N/A Narishige PC-100 Puller Narishige Group N/A (Continued on next page) Cell Reports 42, 112976, August 29, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Borosilicate Glass Capillaries Warner Instruments 64–0772 470-nm LED pE-100 Cool LED pE-100 Contemporary Ortho-Jet Powder (Bilateral Cannula) Lang Dental Ref. 1530 Ortho-Jet Liquid (Bilateral Cannula) Lang Dental Ref. 1304 Dental Cement (Fiber Photometry) Unifast Trad N/A Vetbond 3M 1469Sb ICV Bilateral Internal Cannula P1 Technologies 8IC235ISPCXC ICV Bilateral Guide Cannula P1 Technologies 8IC235G10XXC ICV Bilateral Dummy Cannula P1 Technologies 8IC235DCSPCC Bilateral Cannula Dust Cap P1 Technologies 8K0000303DC1 Stereotaxic frame (Bilateral Cannula/ Optogenetic Release): Drill and Microinjector Robot, Mouse/Rat Stoelting SKU: 51705 Stereotaxic frame (Fiber Photometry) Kopf Instruments Model 942 1 mL Neuros syringe Hamilton 65458–01 5 mL Removable Needle Syringe Hamilton #7634-01 Syringe Pump Stoelting #53311 miniature screws Antrin Miniature Specialities AMS90/1B Fiber Optic Cannula (200 mm core, 0.39 NA) ThorLabs CFMC12L05 473-nm Laser Ready Lasers MBL-III-473-100mW Noise Eeater ThorLabs NEL01 Kinematic Flourescence Filter Cube ThorLabs DFM1 Dual-Edge Dichoric Mirror Chroma ZT488/561rpc FC/PC Fiber Coupler Thorlabs PAF2-A4A Fiber Patchcord (200 mm core, 0.39 NA) ThorLabs M72L05 Fiberoptic Rotary Joint Doric Lenses FRJ_1x1_FC-FC Mating Sleeve Precision Fiber Products SM-CS1140S Dual-band Emission Filter Chroma ZET488/561m Fiber Coupler ThorLabs PAF2S-11A Anti-reflection-coated Fiber ThorLabs M200L02S-A Spectrometer Ocean Insight QE Pro BX63 Upright Microscope Olympus N/A CMS GmbH CMOS fitted Microscope Leica Microsystems N/A LSM 800 Confocal Microscope Zeiss N/A Waveform Generator Sanworks Pulse Pal v2 Blackfly S USB3 Camera FLIR N/A



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    Image Search Results


    Double-labeling immunohistochemical analysis of FAM159B and insulin (A–C), glucagon (D–F), and somatostatin-14/28 (SST) (G–I) expression in the human, rat, and mouse pancreas. Labeling for FAM159B was visualized using Cy3-conjugated anti-rabbit antibody (red) and for insulin, glucagon, or SST using Alexa Fluor-488-conjugated anti-mouse or anti-rat antibody (green); overlapping expression is shown in orange/yellow color. Scale bar: 100 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Expression of FAM159B in Humans, Rats, and Mice: A Cross-species Examination

    doi: 10.1369/00221554241262368

    Figure Lengend Snippet: Double-labeling immunohistochemical analysis of FAM159B and insulin (A–C), glucagon (D–F), and somatostatin-14/28 (SST) (G–I) expression in the human, rat, and mouse pancreas. Labeling for FAM159B was visualized using Cy3-conjugated anti-rabbit antibody (red) and for insulin, glucagon, or SST using Alexa Fluor-488-conjugated anti-mouse or anti-rat antibody (green); overlapping expression is shown in orange/yellow color. Scale bar: 100 µm.

    Article Snippet: For double-labeling experiments, samples were incubated overnight at 4°C with anti-FAM159B HPA011778 antibody together with monoclonal mouse anti-insulin antibody (1:100; Abcam, Cambridge, MA), monoclonal mouse anti-glucagon antibody (1:500; Sigma-Aldrich, St. Louis, MO), or monoclonal rat anti-SST-14/28 antibody (1:300; Abcam).

    Techniques: Labeling, Immunohistochemical staining, Expressing

    Double-labeling immunohistochemical analysis of FAM159B and somatostatin-14/28 (SST) expression in Langerhans islets from lean and obese control and ciprofibrate-treated Zucker rats. Labeling for FAM159B was visualized using Cy3-conjugated anti-rabbit antibody (red; A, D, G, J) and for SST using Alexa Fluor-488–conjugated anti-rat antibody (green; B, E, H, K); overlapping expression is shown in orange/yellow color (C, F, I, L). Scale bar: 100 µm.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Expression of FAM159B in Humans, Rats, and Mice: A Cross-species Examination

    doi: 10.1369/00221554241262368

    Figure Lengend Snippet: Double-labeling immunohistochemical analysis of FAM159B and somatostatin-14/28 (SST) expression in Langerhans islets from lean and obese control and ciprofibrate-treated Zucker rats. Labeling for FAM159B was visualized using Cy3-conjugated anti-rabbit antibody (red; A, D, G, J) and for SST using Alexa Fluor-488–conjugated anti-rat antibody (green; B, E, H, K); overlapping expression is shown in orange/yellow color (C, F, I, L). Scale bar: 100 µm.

    Article Snippet: For double-labeling experiments, samples were incubated overnight at 4°C with anti-FAM159B HPA011778 antibody together with monoclonal mouse anti-insulin antibody (1:100; Abcam, Cambridge, MA), monoclonal mouse anti-glucagon antibody (1:500; Sigma-Aldrich, St. Louis, MO), or monoclonal rat anti-SST-14/28 antibody (1:300; Abcam).

    Techniques: Labeling, Immunohistochemical staining, Expressing, Control